Search results for "Corneal wound"

showing 3 items of 3 documents

NGF and TGF-beta mRNA expression during pregnancy in a rat corneal wound healing model.

2003

Background Growth factors seem to play a major role in corneal wound healing and TGF-beta seems to be associated with abnormal healing after corneal surgical procedures. Few studies have analysed the role of NGF and TGF-beta on corneal wound healing during pregnancy. The aim of the present study was to create an animal model to evaluate the expression of NGF and TGF-betas during corneal wound healing in two groups: control and pregnant rats. Methods Corneal mRNA for NGF and the three isoforms of TGF-beta were analysed by RT-PCR, in a time-course experiment on different days after epithelial wounding (2, 7, 14 days) in pregnant and control groups Results The results show high corneal mRNA ex…

Gene isoformPathologymedicine.medical_specialtyMrna expressionAndrologyCorneaRats Sprague-DawleyPregnancyTransforming Growth Factor betaCorneaNerve Growth FactormedicineAnimalsRNA MessengerMessenger RNAPregnancyWound Healingbusiness.industrymedicine.diseaseeye diseasesRatsOphthalmologymedicine.anatomical_structureCorneal woundPregnancy AnimalFemalesense organsWound healingbusinessOptometryTransforming growth factorCorneal InjuriesClinicalexperimental optometry
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Effects Of COOH-terminal tripeptide alpha-MSH (11-13) on corneal epithelial wound healing:role of nitric oxide

2006

It is known that alpha-melanocyte stimulating hormone (alpha-MSH) may exert anti-inflammatory effects and facilitate reparative processes in different tissues. The effective message sequence of alpha-MSH resides in the COOH-terminal tripeptide alpha-MSH(11-13). This study was undertaken to investigate the effects of topical administration of the COOH-terminal tripeptide sequence of alpha-MSH (alpha-MSH(11-13), KPV) on corneal epithelial wound healing in rabbits and the possible role of nitric oxide (NO) in these effects. The whole corneal epithelium was denuded in both eyes by mechanical abrasion. The area of the corneal epithelial defect was stained with fluorescein, photographed, and then…

MaleNitroprussideMelanocyte-Stimulating Hormonemedicine.medical_treatmentCorneal abrasionRabbitPharmacologyKPVNitric OxideNitric oxideCellular and Molecular Neurosciencechemistry.chemical_compoundPeptide Fragmentα-MSHmedicineAnimalsEnzyme InhibitorNitric Oxide DonorsMelanocyte-Stimulating HormonesFluoresceinEnzyme InhibitorsSalineCells CulturedCorneal epitheliumCell ProliferationEpithelial CellWound HealingbiologyDose-Response Relationship DrugAnimalcorneal wound healingEpithelium CornealEpithelial CellsNitric Oxide Donormedicine.diseaseSensory SystemsPeptide FragmentsNitric oxide synthaseOphthalmologymedicine.anatomical_structureNG-Nitroarginine Methyl EsterchemistryBiochemistrybiology.proteinSodium nitroprussideRabbitsWound healingmedicine.drug
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Hyaluronic acid and beta cyclodextrins films for the release of corneal epithelial cells and dexamethasone

2016

In this work we prepared hydrogels based on hyaluronic acid and β-cyclodextrins to sustain the release of both corneal epithelial cells and dexamethasone. This steroid is administered as eye drops several times per day to reduce the risk of rejection in the post operative period after the cornea transplantation and cell release techniques. Hydrogels were produced by crosslinking an amino derivative of hyaluronic acid, with the divinyl sulfone derivative of β-cyclodextrins, this last employed as a crosslinker and solubilizing agent. Drug release studies revealed that dexamethasone containing samples are able to extend the release of this drug for at least five days. Biological studies, condu…

Materials Chemistry2506 Metals and AlloysPolymers and Plasticsmedicine.medical_treatmentCellBeta-CyclodextrinsCell release systemmacromolecular substances02 engineering and technologyPharmacology010402 general chemistry01 natural sciencesDexamethasoneSteroidCorneachemistry.chemical_compoundCorneaHyaluronic acidMaterials ChemistrymedicineCorneal woundHumansHyaluronic acid hydrogelHyaluronic AcidDexamethasoneCells CulturedDrug CarriersPolymers and Plasticbeta-CyclodextrinsOrganic Chemistrytechnology industry and agricultureEpithelial CellsHydrogelsAnatomy021001 nanoscience & nanotechnologyeye diseases0104 chemical sciencesTransplantationDrug Liberationmedicine.anatomical_structurechemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogelssense organs0210 nano-technologymedicine.drug
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